Accelerating personalized medicine.

Cytogenetic analysis of CTCs provides information regarding diagnosis, prognosis and therapeutic surveillance of cancer patients. We have developed DNA Fluorescence in situ Hybridization (FISH) assays to cytogenetically analyze CTCs downstream of enrichment from whole blood of patients. Isolated cells are first immunophenotyped to identify CTCs and then CTCs are relocated after target-specific FISH analysis has been performed.

      HER-2                   PTEN
      c-Met                      EGFR
      AR                          TMPRSS2-ERG
      EML4-ALK             IGF1-R
      CK2α

Work flow for CTC identification for FISH and representative immunofluorescence images of CTC immunophenotyping based on Cytokeratin, CD45 and DAPI staining.