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Translating every cell’s story.
ApoCell’s CTC molecular profiling services detect the genetic and molecular changes unique to each patient. Our scientists simultaneously characterize Circulating Tumor Cells (CTCs) or Circulating Endothelial Cells (CECs) for single nucleotide polymorphisms (SNPs), gene translocations, rearrangements, copy number alterations, and changes in mRNA or microRNA expression.
Gene Expression Profiling
RNA isolation from Circulating Tumor Cells (CTCs), Circulating Endothelial Cells (CECs), or Peripheral Blood Mononuclear Cells (PBMCs)
RNA quality control using Agilent BioAnalyzer®
Single cell RNA isolation, end-point RT-PCR, quantitative RT-PCR
Gene expression assays using TaqMan® quantitative RT-PCR
Gene expression assays using SYBR-Green quantitative RT-PCR
Gene expression profiling using TaqMan® Custom and Gene Signature Array Microfluidic Cards and Plates
Illumina Human HT-12 Expression BeadChip Arrays
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Cytogenetic Abnormalities (FISH)
Custom Fluorescence In Situ Hybridization (FISH) in CTCs, CECs, FFPE, bone, or frozen tissue
Vysis® FISH assays in CTCs, CECs, FFPE, bone, or frozen tissue
PathVysionâ„¢ for accessing gene amplification in breast cancer
UroVysionâ„¢ aids in the diagnosis and monitoring recurrence in bladder cancer
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Mutation Profiling
Isolation of genomic DNA from Circulating Tumor Cells (CTCs), Circulating Endothelial Cells (CECs), Peripheral Blood Mononuclear Cells (PBMCs), plasma, serum, FFPE, or frozen tissue
PCR amplification and DNA Sequencing
Sequenom® SNP Genotyping
TaqMan® SNP Genotyping
DxS® (Qiagen) Mutation Assays (EGFR29, KRAS, BRAF, BCR-ABL, PI3K)
Custom Allele-Specific PCR for SNP detection
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